Glial-Derived Neurotrophic Factor Human Recombinant, GDNF Human, 10ug

CAT No.BT-CYT-305

Glial-Derived Neurotrophic Factor Human Recombinant, GDNF Human, 10ug

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Amino acid sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Pro-Asp-Lys.

Biological Activity
The ED50, calculated by the dose-dependant dopamin uptake in rat mesencephalic cultures was found to be 5-10 ng/ml, corresponding to a specific activity of 100,000-200,000Units/mg.

Description
Glial derived Neurotrophic Factor Human Recombinant produced in E.Coli is a homodimer, non-glycosylated, polypeptide chain containing 2 x 135 amino acids and having a total molecular mass of 30,360 Dalton.
GDNF is purified by proprietary chromatographic techniques.

GDNF was lyophilized after dialysis against 10mM sodium citrate and 150mM NaCl.

Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

Purity
Greater than 98.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility
It is recommended to reconstitute the lyophilized Glial Derived Neurotrophic Factor in sterile 18M¦¸-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source
Escherichia Coli.

Stability
Lyophilized Glial-derived Neurotrophic Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution GDNF should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms
ATF1, ATF2, HFB1-GDNF, GDNF.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.