Glutathione S-Transferase Recombinant, GST, 50ug

CAT No.BT-ENZ-393

Glutathione S-Transferase Recombinant, GST, 50ug

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Sequence
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPR.

Applications
GST can be used for protein-protein interactions assay and protein-DNA interactions assay.

Biological Activity
0.5-2.5 units/mg (please enquire for specific batch value). A unit is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

Recombinant Glutathione S-Transferase full length protein (1-224a.a.) expressed in E.coli, having a molecular mass of 26kDa. GST was isolated from an E. colistrain that carries the coding sequence for Schistosoma japonicum GST under the control of a T7 promoter.
The GST is purified by proprietary chromatographic techniques.

Formulation
GST supplied in Phosphate Buffered Saline pH 7.4.

Physical Appearance
Sterile Filtered clear solution.

Purity
Greater than 95% as determined by SDS-PAGE.

Source
Escherichia Coli.

Stability
Store vial at -20¡ãC to -80¡ãC. When stored at the recommended temperature, this protein is stable for 12 months.
Please prevent freeze-thaw cycles.

Synonyms
Glutathione S-Transferase, GST, Glutathione S-transferase class-mu 28 kDa isozyme, GST 28, EC 2.5.1.18, Sj28GST, Sj28 antigen.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.