Granulocyte-Colony Stimulating Factor Human Recombinant, G CSF Human, 10ug

CAT No.BT-CYT-220

Granulocyte-Colony Stimulating Factor Human Recombinant, G CSF Human, 10ug

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Amino acid sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Thr-Pro-Leu-Gly.

Biological Activity
The ED50, calculated by the dose-dependant proliferation of murine NFS-60 indicator cells (measured by 3H-thymidine uptake) is < 0.1 ng/ml, corresponding to a Specific Activity of 100,000,000 IU/mg.

Description
Granulocyte Colony Stimulating Factor Human Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 175 amino acids and having a molecular mass of 18.8 KD.
G-CSF is purified by proprietary chromatographic techniques.

G-CSF was lyophilized after extensive dialysis against 10mM sodium acetate buffer pH= 4.

Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

Protein content
G-CSF quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 0.815 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

2. Analysis by RP-HPLC, using a calibrated solution of GCSF as a Reference Standard.

Purity
Greater than 98.0% as determined byAnalysis by RP-HPLC.
Analysis by SDS-PAGE.

Solubility
It is recommended to reconstitute the lyophilized Granulocyte Colony Stimulating Factor in sterile 20mM AcOH not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source
Escherichia Coli.

Stability
Lyophilized Granulocyte Colony Stimulating Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution GCSF should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms
CSF-3, MGI-1G, GM-CSF beta, Pluripoietin, Filgrastim, Lenograstim, G-CSF, MGC45931, GCSF.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.