Granulocyte Macrophage-Colony Stimulating Factor Human Recombinant, GM CSF Human, 10ug

CAT No.BT-CYT-221

Granulocyte Macrophage-Colony Stimulating Factor Human Recombinant, GM CSF Human, 10ug

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Amino acid sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Ala-Arg-Ser.
N-terminal methionine has been completely removed enzymatically.

Biological Activity
The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is < 0.1 ng/ml, corresponding to a Specific Activity of 11,100,000 IU/mg.

Description
Granulocyte Macrophage Colony Stimulating Factor Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids and having a molecular mass of 14477 Dalton. GM-CSF is purified by proprietary chromatographic techniques.

GM-CSF was lyophilized after extensive dialysis against 2mM sodium phosphate buffer pH= 7.4¡À0.1.

Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

Protein content
GM-CSF quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 0.963 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GEN computer analysis program of protein sequences (IntelliGenetics).

2. Analysis by RP-HPLC, using a standard solution of GM-CSF as a Reference Standard.

Purity
Greater than 98.0% as determined by1. Analysis by RP-HPLC.
2. Analysis by SDS-PAGE.

References
1.
Title: Disease activity in systemic lupus erythematosus is associated with an altered expression of low-affinity Fc gamma receptors and costimulatory molecules on dendritic cells.
Publication: Immunology. 2009 Nov;128(3):334-41. PMID: 2770681.
Link: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2770681/
Applications: IL-4 and GM-CSF were used to obtain monocyte-derived Dendritic cells(DC) from Human peripheral blood mononuclear cells (PBMCs).

2.
Title: Generation of Novel Bone Forming Cells (Monoosteophils) from the Cathelicidin-Derived Peptide LL-37 Treated Monocytes.
Publication:  PLoS One. 2010; 5(11): e13985. Published online 2010 November 15.
Link: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2981577/?tool=pmcentrez
Applications: GM-CSF was used to differentiate monocytes into macrophage and to Monocyte-derived dendtritic cells (DCs) from Peripheral blood mononuclear cells (PBMCs).
Osteoclasts, which are derived from monocytes by the action of macrophage colony-stimulating factor (M-CSF) and receptor activator of nuclear factor ?B ligand (RANKL). Osteoclast were differentiated from monocytes in the presence of RANKL and M-CSF (both at 25 ng/mL).
And also for cytokine analysis.

3.
Title:AIMP1/p43 protein induces the maturation of bone marrow-derived dendritic cells with T helper type 1-polarizing ability.
Publication: J Immunol. 2008 Mar 1;180(5):2894-902. PMID: 18292511.
Link: http://www.ncbi.nlm.nih.gov/pubmed?term=18292511
Applications: Generation of bone marrow DCs.

4.
Title:Sphingosine kinase regulates the rate of endothelial progenitor cell differentiation.
Publication: Blood. 2009 Feb 26;113(9):2108-17. Epub 2008 Dec 24. PMID: 19109558. PMID: 18292511.
Link: http://www.ncbi.nlm.nih.gov/pubmed?term=19109558
Applications: BM cells at 10,000 were plated in 0.3% agar culture medium containing GM-CSF (100 ng/ml, was used with other cytokines for mouse progenitor cell isolation, culture and colony forming assay.

Solubility
It is recommended to reconstitute the lyophilized Granulocyte Macrophage Colony Stimulating Factor in sterile 18M¦¸-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source
Escherichia Coli.

Stability
Lyophilized Granulocyte Macrophage Colony Stimulating Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution GMCSF should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms
CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, Molgramostin, Sargramostim, MGC131935, MGC138897.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.