Granulocyte Macrophage-Colony Stimulating Factor Rhesus Macaque Recombinant, GM CSF Monkey, 10ug

CAT No.BT-CYT-720

Granulocyte Macrophage-Colony Stimulating Factor Rhesus Macaque Recombinant, GM CSF Monkey, 10ug

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Amino acid sequence
APARSPSPGT QPWEHVNAIQ EARRLLNLSR DTAAEMNKTV EVVSEMFDLQ EPSCLQTRLE LYKQGLQGSL TKLKGPLTMM ASHYKQHCPP TPETSCATQI ITFQSFKENL KDFLLVIPFD CWEPVQE.

Biological Activity
The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is < 0.1 ng/ml, corresponding to a Specific Activity of 10,000,000 IU/mg.

Description
Granulocyte Macrophage Colony Stimulating Factor Monkey Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids and having a molecular mass of 14.4 kDa. GM-CSF is purified by proprietary chromatographic techniques.

GM-CSF was lyophilized after extensive dialysis against 1x PBS pH= 7.4¡À0.1.

Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

Purity
Greater than 95.0% as determined by: (a) Analysis by HPLC. (b) Analysis by SDS-PAGE.

Solubility
It is recommended to reconstitute the lyophilized GMCSF in sterile 18M¦¸-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source
Escherichia Coli.

Stability
Lyophilized GMCSF although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution GMCSF should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Synonyms
CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, Molgramostin, Sargramostim, MGC131935, MGC138897.

Usage
TSZGENE's products are furnished for?LABORATORY RESEARCH?USE?ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.