Growth Hormone Chicken Recombinant, GH Chicken, 500ug

CAT No.BT-CYT-430

Growth Hormone Chicken Recombinant, GH Chicken, 500ug

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Amino acid sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Thr-Phe-Pro-Ala.

Biological Activity
GH Chicken Recombinantis fully biologically active in homologous assays and in PDF-P1 3B9 cells stably transfected with rabbit GH receptors.

Description
Growth Hormone Chicken Recombinant (cGH) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 191 amino acids with an additional Ala at its N-terminus and having a molecular mass of 22255 Dalton.
GH Chicken recombinant is purified by proprietary chromatographic techniques.

The protein was lyophilized from a concentrated (1mg/ml) solution with 0.3% NaHCO3 adjusted to pH 8.

Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

Protein content
Protein quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 0.75 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0 This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

2. Analysis by RP-HPLC, using a calibrated solution of GH as a Reference Standard.

Purity
Greater than 99.0% as determined by:
(a) Analysis by SDS-PAGE gel.
(b) Analysis by SEC-HPLC.

Solubility
It is recommended to reconstitute the lyophilized GH Chicken Recombinant in sterile water or 0.4% NaHCO3 adjusted to pH 8-9, not less than 100ug/ml and not more than 3 mg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.

Source
Escherichia Coli.

Stability
Lyophilized GH Chicken although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution at > 0.1 mg/ml and filter sterilization GH can be stored at 4¡ãC for several weeks. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is recomended.
Please prevent freeze-thaw cycles.

Synonyms
GH1, GH, GHN, GH-N, hGH-N,Pituitary growth hormone, Growth hormone 1, Somatotropin.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY.They may not be used as drugs,agricultural or pesticidal products, food additives or household chemicals.

Amino acid sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Phe-Pro-Ala.