Heat Shock Protein 65 Mycobacterium Tuberculosis Recombinant, HSP65 Mycobacterium, 10ug

CAT No.BT-HSP-065

Heat Shock Protein 65 Mycobacterium Tuberculosis Recombinant, HSP65 Mycobacterium, 10ug

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Amino Acid Sequence
HHHHHHGSAKTIAYDEEARRGLERGLNALADAVKV
TLGPKGRNVVLEKKWGAPTITNDGVSIAKEIELEDPY
EKIGAELVKEVAKKTDDVAGDGTTTATVLAQALVRE
GLRNVAAGANPLGLKRGIEAVEKVTETLLKGAKEVET
KEQIAATAAISAGDQSIGDLIAEAMDKVGNEGVITV
EESNTFGLQLELTEGMRFDKGYISGYFVTDPERQEAV
LEDPYILLVSSKVSTVKDLLPLLEKVIGAGKPLLIIEDVEGE
ALSTLVVNKIRGTFKSVAVKAPGFGDRRKAMLQDMAIL
TGGQVISEEVGLTLENADLSLLGKARKVVVTKDETTIVE
GAGDTDAIAGRVAQIRQEIENSDSDYDREKLQERLAKL
AGGVAVKAGAATEVELKERKHRIEDAVRNAKAAVEEGIV
AGGGVTLLQAAPTLDELKLEGDEATGANIVKVALEAPL
KQIAFNSGLEPGVVAEKVRNLPAGHGLNAQTGVYEDLLA
AGVADPVKVTRSALQNASIAGLFLTTEAVVADKPEKEKASVPGGGDMGGMDF.

Description
Recombinant Mycobacterium Tuberculosis HSP65 is produced in E.coli and has a Mw of 57.4 kDa. The HSP65 protein is fused to His-Tag at N-Terminus and purified by stabdard chromatography techniques.

Formulation
The HSP65 protein was lyophilized from a concentrated (1mg/ml) solution containing 10mM Na-phosphate pH-7.4, 130mM NaCl and 2.5mM KCl.

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity
Greater than 95.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility
It is recommended to reconstitute the lyophilized HSP-65 in sterile 18M¦¸-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source
Escherichia Coli.

Stability
Lyophilized HSP65 although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution HSP65 should be stored at 4¡ãC between 2-7 days and for future use below
-18¡ãC. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms
Protein Cpn60-2, groEL protein-2, 65 kDa antigen, Heat shock protein 65, Cell wall protein A, Antigen A, groL2, groEL-2.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.