Hepatitis D Virus Mouse Antibody, HDV Antibody, 50ug

CAT No.BT-ANT-158

Hepatitis D Virus Mouse Antibody, HDV Antibody, 50ug

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Antigen used for Immunizations
The hepatitis virus delta antigen-coding fragment (isolateTW2576) was inserted into pPinPoint plasmid and the expressed recombinant was used for mouse immunization. After cell fusion, the hybridomas obtained were screened for antibody reacting withthe delta antigen fused in different format (MBP-25L) and tested negative for other fusion proteins.

Concentration
HDV Antibody-HP6A1 is supplied at the concentration of 1 ug/?l.

Immunofluorescence
Typical working dilution on frozen sections 1:200. 60 minutes primary antibody incubates at 25¡ãC.
2.

Reconstitute in 18M¦¸-cm H2O to a concentration of 1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at
<-20¡ãC.
If more dilute solution required, it is suggested that they be made in a buffered solution containing carrier protein such as PBS with 0.1% BSA.

Reconstitutions on use
1.

Specificity
Mouse monoclonal antibody against the amino acid residues 4-10 (SESKRNR) of HDAg (isolate 25).

Storage and Stability
Store unopened antibody at 4¡ãC or lower. Under these conditions, there is no significant loss in product performance one year from the purchase date. The reconstituted antibody is stable for at least two months when stored at 4¡ãC. For long term storage, it is recommended that aliquots of the antibody are frozen at -20¡ãC (frost-free freezers are not recommended). Repeated freezing and thawing must be avoided. Prepare working dilutions on the day of use.

Storage Buffer
The ammonium sulfate-precipitated ascites fluid is kept in PBS with 1% BSA and then lyophilized.

Type
Mouse antibody Monoclonal.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Western blotting
Recommended: working dilution onWestern blotting 1:500. 60 minutes primary antibody incubates at 25¡ãC.