Hepatocyte Growth Factor Human Recombinant CHO, HGF Human CHO, 10ug

CAT No.BT-CYT-251

Hepatocyte Growth Factor Human Recombinant CHO, HGF Human CHO, 10ug

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Amino acid sequence
Agrees with the sequence of native human HGF.

Biological Activity
The ED50, calculated by the dose-dependant proliferation of monkey 4MBr-5 indicator cells was found to be 20-40ng/ml corresponding to a Specific Activity of 25,000-50,000IU/mg.

Description
Hepatocyte Growth Factor Human Recombinant produced in CHO is a heterodimer, non-glycosylated, polypeptide chain consisting an ?-chain of 463 amino acids and b-chain of 234 having a total molecular mass of 75kDa.
The HGF is purified by proprietary chromatographic techniques.

The protein was lyophilized from a concentrated (1mg/ml) solution containing Phosphate-Buffered Saline with 0.02% Tween 80, pH 7.4.

Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

Protein content
Protein quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 1.83 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

2. Analysis by RP-HPLC, using a calibrated solution of HGF as a Reference Standard.

Purity
Greater than 97.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility
It is recommended to reconstitute the lyophilized Hepatocyte Growth Factor in sterile 18M¦¸-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source
Chinese Hamster Ovary Cells.

Stability
Lyophilized Hepatocyte Growth Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution HGF should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms
Scatter Factor (SF), Hepatopoietin (HPTA), HGF.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.