Hirudin Recombinant, Hirudin, 10ug

CAT No.BT-PRO-362

Hirudin Recombinant, Hirudin, 10ug

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Biological Activity
The biological activity is measured using chromogenic assay, 1 unit is defined as the amount of Hirudin that neutralizes 1 unit of the WHO preparation 89/588 of thrombin. The specific activity was found to be 16,000ATU/mg protein.

Description
Recombinant Hirudin is derived from yeast and the polypeptide chain contains 65 amino acids and its Mw is 6979.5 dalton which is identical to natural Hirudin except for the substitution of leucine for isoleucine at the N-terminal end of the molecule and the absence of a sulfate group on the tyrosine at position 63.
The Recombinant Hirudin is purified by proprietary chromatographic techniques.

Formulation
Each mg of protein was lyophilized from a sterile solution containing no 20mM PBS pH-7 and 2% mannitol.

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity
Greater than 95.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility
It is recommended to reconstitute the lyophilized Hirudin in sterile 18M¦¸-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source
Pichia Pastoris.

Stability
Lyophilized Hirudin although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution Hirudin should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.