Human Slit2 ELISA Kit , 96T

Human Slit2 ELISA Kit , 96T

Assay range: 0.15 – 40 ng/ml                      

Purpose

This kit is used for the quantitative determination the concentrations of Human Slit2 in human serum, plasma, cell culture supernatants, and other biological fluids.

Principle of the assay

The kit assays human Slit2 in the samples by the method of double-antibody sandwich ELISA. The solid-phase antibody is prepared by purified human Slit2 antibody which is pre-coated onto a microplate. After loading of samples, the Slit2 in the standards or samples bind with the coated antibody. Following by adding biotinylated-Slit2 antibody, the HRP-Streptavidin enzyme conjugate has been added into wells. After washing to remove any unbound antibody-enzyme reagent, the tetramethylbenzidine (TMB) substrate is added. TMB substrate produces blue color when catalyzing by the HRP enzyme. After reaching the desired color intensity, the reaction is terminated by addition of an acidic Stop solution which changes the solution color from blue to yellow. Determining the absorbance (OD Value) at wave length 450 nm by the microwell reader, and the concentration of Slit2 can be calculated according to standard curve.

Kit Performance                                   

1.       Sensitivity: less than 0.15 ng/ml.

2.       Specificity: simultaneous detection of recombinant or natural human Slit2 without cross-reaction with other cytokines.

3.       Repeatability: both inter-assay and intra-assay were less than 12 %.

*Lead time of this kit is 1-2 weeks. Shipped by FedEx air. 
*Storage: 2 - 8 C. Shelf Time: 6 months.
*Kit Experimental Time:< 2 hs

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Important note: Products are intended for laboratory or manufacture use and are not to be used for diagnosis or any other purpose, which include but are not limited to, unauthorized commercial uses, ex vivo or in vivo therapeutic uses. Not for Human or Animal Consumption.