Interferon-alpha 2b 40kd-Pegylated Human Recombinant, IFN a 2b Human, 40 kd PEG, 10ug

CAT No.BT-CYT-019

Interferon-alpha 2b 40kd-Pegylated Human Recombinant, IFN a 2b Human, 40 kd PEG, 10ug

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Amino acid sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Cys-Asp-Leu-Pro.

Biological Activity

The specific activity as determined in a viral resistance assay using VSV-WISH cells was found to be 1,000,000 IU/mg.

Description
Interferon-a 2b Human Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 19269 Dalton. The Pegylated IFN-a 2b is produced by attaching 40kDa NHS-mPEG to the primary amino acid of IFN alpha-2b.
Interferon-a 2b is purified by proprietary chromatographic techniques.

IFN-a 2b was lyophilized after extensive dialysis against 10mM Phosphate buffer, pH7.0, 150mM NaCl, 0.01% EDTA and 0.01% Tween 80 buffer.

Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

Purity
Greater than 98.0% as determined by:
a) Analysis by RP-HPLC.
b) Analysis by SDS-PAGE.

Solubility
It is recommended to reconstitute the lyophilized Interferon alpha 2b in sterile 18M?-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source
Escherichia Coli.

Stability

Lyophilized Interferon although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution the Pegylated IFN alpha 2b should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms
Interferon alpha 2b, IFNA, INFA2, MGC125764, MGC125765.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.