Interferon-Beta 1b Human Recombinant, IFN b 1b Human, 10ug

CAT No.BT-CYT-234

Interferon-Beta 1b Human Recombinant, IFN b 1b Human, 10ug

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Amino acid sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Tyr-Asn-Leu-Leu.

Biological Activity
The specific activity as determined in a viral resistance assay (human "Wish" cell line and VSV virus or the monkey VERO cell line with EMCV virus) was found to be  32MIU/mg.

Description
Interferon beta 1b Human Recombinant produced in E.Coli is a single, non-glycosylated mutein (variant form) of human Interferon beta-1b polypeptide chain containing 165 amino acids and having a molecular mass of 18510.86 Dalton.
The IFN-beta gene was cloned from human fibroblasts and altered to substitute Serine for the Cysteine residue found at position 17.
IFN beta-1b is purified by proprietary chromatographic techniques.

Lyophilized from 1mg/ml solution containing 50mg Human Albumin & 50mg dextrose.

Protein content
Protein quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 1.493 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

2. Analysis by RP-HPLC, using a calibrated solution of IFN-beta as a Reference Standard.

Purity
Greater than 98.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility
It is recommended to reconstitute the lyophilized Interferon beta-1b in sterile 18M¦¸-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source
Escherichia Coli.

Stability
Lyophilized Interferon-beta 1b although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution IFNB 1b should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC.
Please prevent freeze-thaw cycles.

Synonyms
Leukocyte interferon, B cell interferon, Type I interferon, IFNB1, IFB, IFF, IFNB, IFN-b 1b, MGC96956.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.