Interleukin-11 Human Recombinant, Pichia, IL 11 Human, Pichia, 10ug

CAT No.BT-CYT-013

Interleukin-11 Human Recombinant, Pichia, IL 11 Human, Pichia, 10ug

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Amino acid sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Gly-Pro-Pro-Pro-Gly.

Biological Activity

The ED50 as determined by the dose-dependent stimulation of the proliferation of murine 7TD1 was found to be less then 0.2ng/ml, corresponding to a Specific Activity of 8,000,000 IU/ mg.

Description

IL11 Human Recombinant produced in Pichia Pastoris is a single, non-glycosylated, Polypeptide chain containing 177 amino acids (it differs from the 178 amino acid length of the native IL11 only in lack of the N-terminal praline residue) and having a molecular mass of 19kDa.
The IL11 is purified by proprietary chromatographic techniques.

IL11 was lyophilized after extensive dialysis against 20mM PB, pH7.0 and 2% Glycine buffer.

Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

Purity
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility

It is recommended to reconstitute the lyophilized Interleukin -11 in sterile 18M?-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source
Pichia Pastoris.

Stability
Lyophilized IL11 although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution IL11 should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC.
Please prevent freeze-thaw cycles.

Synonyms

Interleukin-11, IL-11, Adipogenesis inhibitory factor, AGIF, Oprelvekin, IL11.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.