Interleukin-12 Human Recombinant, IL 12 Human, 10ug

CAT No.BT-CYT-362

Interleukin-12 Human Recombinant, IL 12 Human, 10ug

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Amino acid sequence
The sequence of the first five N-terminal amino acids was determined and found to be Arg-Asn-Leu-Pro-Val at the p35 subunit and Ile-Trp-Glu-Leu-Lys at the p40 subunit.

Biological Activity
Measured by its ability to stimulate the proliferation of PHA-activated human T lymphoblasts. The ED50 for this effect is 0.3-0.5 ng/ml corresponding to a specific activity of 2-3.3MU/mg.

Description
Interleukin-12 Human Recombinant produced in CHO is a glycosylated disulfide linked heterodimeric polypeptide containing 503 amino acids and having a molecular weight of 75 kDa comprised of disulfide-bonded 35 kDa (p35) and 40 kDa (p40) subunits.
The IL-12 is purified by proprietary chromatographic techniques.

Lyophilized from a 0.2¦Ìm filtered solution (0.5mg/ml) in PBS, pH 7.4.

Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

Purity
Greater than 98% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility
It is recommended to reconstitute the lyophilized Interleukin-12 in sterile 18M¦¸-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source
CHO (Chinese Hamster Ovarian Cells).

Stability
Lyophilized Interleukin12 although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution IL12 should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms
NKSF, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor (CLMF), TSF, Edodekin-alpha, IL-12.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.