Interleukin-13 Mouse Recombinant, IL 13 Mouse, 10ug

CAT No.BT-CYT-375

Interleukin-13 Mouse Recombinant, IL 13 Mouse, 10ug

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Amino acid sequence
The sequence of the first five N-terminal amino acids was determined and found to be Met-Pro-Val-Pro-Arg.

Biological Activity
The ED50 range=2-6 ng/ml corresponding to a specific activity of 0.17-0.5MU/mg, determined by the dose dependent prolifiration of TF-1 cells.

Description
Interleukin-13 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 110 amino acids and having a molecular mass of 12 kDa.
The IL-13 is purified by proprietary chromatographic techniques.

The protein (1mg/ml) was lyophilized with no additives.

Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

Protein content
Protein quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 0.69 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

2. Analysis by RP-HPLC, using a calibrated solution of IL-13 as a Reference Standard.

Purity
Greater than 95% as determined by SDS-PAGE.

Solubility
It is recommended to reconstitute the lyophilized Interleukin 13 in sterile 18M¦¸-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source
Escherichia Coli.

Stability
Lyophilized Interleukin-13 although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution IL13 should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms
Interleukin-13, NC300, ALRH, BHR1, P600, IL-13, IL13.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.