Interleukin-5 Human Recombinant, IL 5 Human, 10ug

Amino acid sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ile-Pro-Thr-Glu.

Biological Activity
The ED50 as determined by the dose-dependant stimulation of the proliferation of TF-1 cells was found to be < 0.15ng/ml, corresponding to a Specific Activity of 6.7MIU/mg.

Description
Interleukin-5 Human Recombinant produced in E.Coli is a dimeric, non-glycosylated polypeptide chain containing 132 amino acids and having a molecular mass of 26522.84 Dalton.
The IL-5 is purified by proprietary chromatographic techniques.

Formulation
Lyophilized from a concentrated (1mg/ml) solution in water containing no additives.

Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

Purity
Greater than 98.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility
It is recommended to reconstitute the lyophilized Interleikin-5 in sterile 18M¦¸-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source
Escherichia Coli.

Stability
Lyophilized Interleukin-5 although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution IL5 should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC.
Please avoid freeze thaw cycles.

Synonyms
EDF, BCDFII, TRF, T-cell replacing factor, Eosinophil differentiation factor, B cell differentiation factor I, IL-5.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Amino acid sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Ile-Pro-Met.

Biological Activity
The ED50 range=0.3-1.0 ng/ml corresponding to a specific activity of 1-3.4MIU/mg as determined by the dose-dependant stimulation of the proliferation of BCL-1 cells.