Interleukin-5 Rat Recombinant, IL 5 Rat, 10ug

Amino acid sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Ile-Pro-Met.

Biological Activity
The ED50 range=0.3-1.0 ng/ml corresponding to a specific activity of 1-3.4MIU/mg as determined by the dose-dependant stimulation of the proliferation of BCL-1 cells.

Description
Interleukin-5 Rat Recombinant produced in E.Coli is a dimeric, non-glycosylated polypeptide chain containing 113 amino acids and having a molecular mass of 13074 Dalton.
The IL-5 is purified by proprietary chromatographic techniques.

Formulation
Lyophilized from a concentrated (1mg/ml) solution in water containing no additives.

Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

Purity
Greater than 95.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility
It is recommended to reconstitute the lyophilized Interleikin-5 Rat in sterile 18M¦¸-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source
Escherichia Coli.

Stability
Lyophilized Interleukin-5 Rat although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution IL5 Rat should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC.
Please avoid freeze thaw cycles.

Synonyms
EDF, BCDFII, TRF, T-cell replacing factor, Eosinophil differentiation factor, B cell differentiation factor I, IL-5.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.