Interleukin-6 Mouse Recombinant, IL 6 Mouse, 10ug

CAT No.BT-CYT-350

Interleukin-6 Mouse Recombinant, IL 6 Mouse, 10ug

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Amino acid sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Phe-Pro-Thr-Ser.

Biological Activity
The ED50 as determined by the dose-dependant induction of 7TD1 cell proliferation,  is 0.018 ng/ml corresponding to a Specific Activity of 55MIU/mg.

Description
Interleukin-6 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 187 amino acids and having a molecular mass of 21709 Dalton.
The IL-6 is purified by proprietary chromatographic techniques.

The protein was lyophilized without any additives.

Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

Purity
Greater than 95.0% as determined by SDS-PAGE.

Solubility
It is recommended to reconstitute the lyophilized Interleukin 6 in 10mM HCl to 0.1-1.0 mg/ml to regain full activity, and can then be further diluted to other aqueous solutions.

Source
Escherichia Coli.

Stability
Lyophilized Interleukin-6 although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution IL6 should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms
IFN-b2, B cell differentiation factor (BCDF), BSF-2, HPGF, HSF, MGI-2, IL-6, Interleukin HP-1, B-cell hybridoma growth factor.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.