Interleukin-6 Porcine Recombinant, IL 6 Porcine, 10ug

CAT No.BT-CYT-399

Interleukin-6 Porcine Recombinant, IL 6 Porcine, 10ug

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Amino acid sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Arg-Leu-Glu.

Biological Activity
The ED50 as determined by the dose-dependant stimulation of B9 cells is < 0.1 ng/ml, corresponding to a Specific Activity of 10MIU/mg.

Description
Interleukin-6 Porcine Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 184 amino acids and having a molecular mass of 21,113 Dalton.
The IL-6 is purified by proprietary chromatographic techniques.

The protein was lyophilized containing no additives.

Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

Protein content
Protein quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 0.581 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

2. Analysis by RP-HPLC, using a standard solution of IL-6 as a Reference Standard.

Purity
Greater than 95.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility
It is recommended to reconstitute the lyophilized Interleukin 6 in sterile 18M¦¸-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source
Escherichia Coli.

Stability
Lyophilized Interleukin-6 although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution IL6 should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms
IFN-b2, B cell differentiation factor (BCDF), BSF-2, HPGF, HSF, MGI-2, IL-6, Interleukin HP-1, B-cell hybridoma growth factor.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.