Interleukin-7 Human Recombinant, Yeast, IL 7 Human, Yeast, 10ug

CAT No.BT-CYT-298

Interleukin-7 Human Recombinant, Yeast, IL 7 Human, Yeast, 10ug

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Amino acid sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Asp-Cys-Asp-Ile-Glu.

Biological Activity
The ED50 as determined by the dose-dependent stimulation of thymidine uptake by murine pre-B cell line 2E8 is < 0.5 ng/mL, corresponding to a specific activity of 2MU/mg.

Description
Interleukin-7 Human Recombinant produced in yeast is a single, glycosylated polypeptide chain containing 152 amino acids and having a molecular mass of 17.4 kDa. The IL-7 is purified by proprietary chromatographic techniques.

Lyophilized from a concentrated (1mg/ml) solution in water containing 20mM phosphate buffer.

Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

Protein content
Protein quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 0.418 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

2. Analysis by RP-HPLC, using a standard solution of IL-7 as a Reference Standard.

Purity
Greater than 98.0% as determined by SDS-PAGE.

Solubility
It is recommended to reconstitute the lyophilized Interleukin -7 in sterile 18M¦¸-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source
Saccharomyces cerevisiae.

Stability
Lyophilized Interleukin-7 although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution IL7 should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms
Lymphopoietin 1 (LP-1), pre-B cell factor, IL-7.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.