Intrinsic Factor Human Recombinant, IF Human, 10ug

CAT No.BT-PRO-375

Intrinsic Factor Human Recombinant, IF Human, 10ug

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Biological Activity
Standard ELISA test (checker-board analysis of positive/negative sera panels), immunodot with anti-IF autoantibody positive patient sera.
Can be used for solid ELISA and fluid phase diagnostic assays, 0.25-0.65ug/ml.
Suitable for biotinylation and iodination.

Description
Intrinsic Factor Human Recombinant produced in baculovirus is a glycosylated, polypeptide chain containing having a molecular mass of 55,000 Dalton.
The Intrinsic Factor is fused to a hexa-histidine at the C-terminus and purified by proprietary chromatographic techniques for removal of bound Vitamin B-12.

Formulation
The protein solution (0.7mg/ml) contains 20mM HEPES pH-8, 100mM NaCl, 20% Glycerol.

Sterile Filtered colorless solution.

Purity
Greater than 95.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Source
Sf9 Insect Cells.

Stability
IF although stable at 10¡ãC for 1 week, should be stored desiccated below -18¡ãC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms
Gastric intrinsic factor, Intrinsic factor, INF, IF, GIF, IFMH, TCN3, Cobalamin/Vitamin B-12 binding transport protein.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.