Kallikrein-11 Human Recombinant, KLK11 Human, 10ug

CAT No.BT-ENZ-511

Kallikrein-11 Human Recombinant, KLK11 Human, 10ug

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Activation
KLK11 is activated in a reaction containing 100¦Ìg/ml Kallikrein 11, 10 ¦Ìg/ml thermolysin, 50mM Tris, 10mM CaCl2, 150mM NaCl, 0.05% Brij 35, pH 7.5 for 15 minutes at 37¡ãC. The thermolysin reaction is stopped by adding EDTA to a final concentration of 50mM.

Amino Acid Sequence
ETRIIKGFEC KPHSQPWQAA LFEKTRLLCG ATLIAPRWLL TAAHCLKPRY IVHLGQHNLQ KEEGCEQTRT ATESFPHPGF NNSLPNKDHR NDIMLVKMAS PVSITWAVRP LTLSSRCVTA GTSCLISGWG STSSPQLRLP HTLRCANITI IEHQKCENAY PGNITDTMVC ASVQEGGKDS CQGDSGGPLV CNQSLQGIIS WGQDPCAITR KPGVYTKVCK YVDWIQETMK NN.

Biological Activity
KLK11 specific activity is greater than 2200 pmoles/min/¦Ìg when measure with 100uM colormetric peptide substrate (D-Val-Leu-Lys-ThioBenzyl ester) and 1 ¦Ìg of activated enzyme. The reaction is carried out in a volume of 100¦ÌL containing 50mM Tris, 1M NaCl, 10mM EDTA, 0.1mM DTNB (5,5¡¯Dithio-bis(2-nitrobenzoic acid)), pH8.5 at 37¡ãC. Cleavage of the peptide substrate can be measured at a wavelength of 405nm and quantified using the extinction coeffiecient 13,260M-1cm-1.

KLK11 Human Recombinant produced in HEK-293 cells is a single, glycosylated, polypeptide chain having a molecular weight of 25.6kDa though on SDS-PAGE migrates at about 41kDa due to the glycosilation. The KLK11 is purified by proprietary chromatographic techniques.

Formulation
The KLK11 protein was lyophilized from a 0.2?m filtered solution containing no additives or preservatives.

Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

Purity
Greater than 95.0% as determined by SDS-PAGE.

Solubility
It is recommended to reconstitute the lyophilized KLK11 in sterile 18M¦¸-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source
HEK293-F Cells.

Stability
Lyophilized KLK11 although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution KLK11 should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Synonyms
kallikrein-related peptidase 11, TLSP, hippostasin, Kallikrein-11, hK11, Hippostasin, Trypsin-like protease, Serine protease 20, PRSS20, MGC33060, EC 3.4.21.

Usage
TSZGENE's products are furnished for?LABORATORY RESEARCH?USE?ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.