Kallikrein-7 Human Recombinant, KLK7 Human, 10ug

CAT No.BT-ENZ-510

Kallikrein-7 Human Recombinant, KLK7 Human, 10ug

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Activation
Recombinant Human KLK7 is activated in a reaction containing 100¦Ìg/ml Kallikrein 7, 10 ¦Ìg/ml thermolysin, 50mM Tris, 10mM CaCl2, 150mM NaCl, 0.05% Brij 35, pH 7.5 for 2 hours at 37¡ãC. The thermolysin reaction is stopped by adding EDTA to a final concentration of 50mM.

Amino acid sequence
EEAQGDKIID GAPCARGSHP WQVALLSGNQ LHCGGVLVNE RWVLTAAHCK MNEYTVHLGS DTLGDRRAQR IKASKSFRHP GYSTQTHVND LMLVKLNSQA RLSSMVKKVR LPSRCEPPGT TCTVSGWGTT TSPDVTFPSD LMCVDVKLIS PQDCTKVYKD LLENSMLCAG IPDSKKNACN GDSGGPLVCR GTLQGLVSWG TFPCGQPNDP GVYTQVCKFT KWINDTMKKH R.

Biological Activity
Recombinant Human KLK7 specific activity is greater than 300pmoles/min/¦Ìg when measure with 10uM fluorogenic peptide substrate (Mca-Arg-Pro-Lys-Pro-Val-Glu-Nval-Trp-Arg-Lys(Dnp)-NH2) and 200ng of activated enzyme. The reaction is carried out in a volume of 100¦ÌL containing 50mM Tris, 150mM NaCl, pH8.5 at 37¡ãC. Cleavage of the peptide substrate can be monitored using excitation and emission wavelengths of 320nm and 405nm, respectively.

KLK7 Human Recombinant produced in HEK-293 cells is a single, glycosylated, polypeptide chain containing 231 amino acids with a molecular weight of 30 kDa. The KLK7 is purified by proprietary chromatographic techniques.

Formulation
The KLK7 protein was lyophilized from a 0.2?m filtered solution containing no additives or preservatives.

Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

Purity
Greater than 95.0% as determined by SDS-PAGE.

Solubility
It is recommended to reconstitute the lyophilized KLK7 in sterile 18M¦¸-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source
HEK293-F Cells.

Stability
Lyophilized KLK7 although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution KLK7 should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Synonyms
kallikrein-related peptidase 7, SCCE, hSCCE, EC 3.4.21.117, hK7, Kallikrein-7, PRSS6, KLK7.

Usage
TSZGENE's products are furnished for?LABORATORY RESEARCH?USE?ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.