Lipase-A Recombinant, Lipase A, 100mg

CAT No.BT-ENZ-473

Lipase-A Recombinant, Lipase A, 100mg

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Application Stability
pH5~10 Unstable at higher than 50¡ãC. Higher activity can be reached in 10%-50% DMSO, isopropyl ether and petroleum ether, 10%-25% ethanol, acetone and isopropanol, 50% activity can be kept in 50% acetone and isopropanol, no activity detected in 50% ethanol.

Biological Activity
580 units/mg powder.

Description
Recombinant Immobilized Serratia marcescens Lipase-A is expressed in E.Coli having a Mw of 65 kDa is purified by standard chromatography techniques.

The protein was lyophilized without additives.

Physical Appearance
Sterile Filtered lyophilized powder.

Purity
Greater than 90% as determined by SDS-PAGE.

Solubility
It is recommended to reconstitute the lyophilized Lipase-A in sterile 18M¦¸-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source
Escherichia Coli.

Stability
Recombinant Lipase-A although stable at room temp for 1 week, should be stored desiccated below -18C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Unit Definition
Lipase-A was assayed using p-nitrophenyl acetate ( pNPA) as a substrate. One unit of lipase activity was defined as the amount of enzyme releasing 1.0 ¦Ìmol of p-nitrophenol per minute.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.