Lipopolysaccaride Human Recombinant, LBP Human, 5ug

CAT No.BT-PRO-538

Lipopolysaccaride Human Recombinant, LBP Human, 5ug

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Characterization
On SDS-PAGE commassie blue stained gel, the 95% purified recombinant protein shows a band at 58 kDa.

Activity
Up to 0.2 ug/ ml LBP mediates binding of FITC-LPS (0.5ug/ml) to CD14+CHO transfectants (FACS).

Description
The Lipopolysaccharide Binding Protein is produced from human LBP transfected CHO-cells in serum free medium. Before transfection the complete human LBP-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR.
The recombinant Human LBP was purified by his-tag with metal affinity purification with Talon and controlled by SDS page.
Attention: His-tag has no protease site and can not be split off.

Recombinant Human LBP was lyophilized from a protein solution (0.25 mg/ml) containing phosphate-buffered saline, pH 7.2.

Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

Solubility
Every 10ug of recombinant human LBP should be reconstituted using 40?l of sterile H2O. The solution can be than diluted with phosphate-buffered saline or other buffers.

Source
Chinese Hamster Ovarian Cells (CHO).

Stability
Lyophilized LBP Human Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution LBP should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC.
Please prevent freeze-thaw cycles.

Synonyms
Lipopolysaccharide-binding protein, LBP, MGC22233.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.