Lysozyme Human Recombinant, Lysozyme Human, 1gr

CAT No.BT-ENZ-362

Lysozyme Human Recombinant, Lysozyme Human, 1gr

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Activity
136,227 Units/mg protein. 1 unit will produce a change of A450 0.001 per minute at pH-6.24 and 25¡ãC, using a suspension of micrococcus lysodeikticus as substrate in a 2.6ml reaction volume.

Description
Recombinant Human Lysozyme is added in lysing bacterial cells to the cell extraction buffer at a range of 0.05-0.5 mg/ml (7,000-70,000 U/ml).
Recombinant Human Lysozyme produced in Plant is a single, non-glycosilated polypeptide chain containing 130 amino acids and a molecular mass of 14 kDa.

Formulation
The Recombinant Lysozyme was lyophilized with no additives.

Recombinant Human Lysozyme is added in lysing bacterial cells to the cell extraction buffer at a range of 0.05-0.5mg/ml (7,000-70,000 U/ml). The common extraction buffer for E. coli cells contains 0.2mg/ml of recombinant Lysozyme in 100mM Tris-HCl, 2mM EDTA, 0.05% Triton X-100, pH 8. The cell paste is suspended in extraction buffer and incubated for at least 15 min at room temperature. Alternatively, Triton X-100 at 1% and PMSF at 1mM can be added separately and the lysis solution is further incubated for at least 15 min at 37 ¡ãC. The lysed cell suspension is clarified by centrifugation at 20,000g for at least 15 min. The extraction conditions and concentration of the Recombinant Human Lysozyme needed for optimal cell lysis and protein recovery depends on various factors and has to be determined empirically.

Physical Appearance
Sterile Filtered white lyophilized powder.

Purity
Greater than 90% as determined by both: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Source
Rice Grain.

Stability
Recombinant Lysozyme although stable at room temperature for 3 weeks, should be stored at 2-8¡ãC.

Synonyms
Lysozyme C, EC 3.2.1.17, LYZ, LZM, 1,4-beta-N-acetylmuramidase C, Lysozyme.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.