Maltose-Binding protein, Mouse Antibody, MBP Antibody, 50ug

CAT No.BT-ANT-177

Maltose-Binding protein, Mouse Antibody, MBP Antibody, 50ug

---

Antigen used for immunization
The malE gene is inserted in a pMAL vector (Maina et al., 1988) and then expressed in E. coli JM109.
The purified recombinant MBP protein was then used for mouse immunization.

Recommendations on use
Immunofluorescence: Typical working dilution on frozen sections 1:200. 60 minutes primary antibody incubates at 25¡ãC.

Specificity
Mouse monoclonal antibody against the amino acid stretch of KDPRIAA (the amino acid residue 339 to 345) of the periplasmic maltose-binding protein (MBP) [Escherichia coli] (Accession #: AAB59056).

Store unopened antibody at 4¡ãC or lower. Under these conditions, there is no significant loss in product performance one year from the purchase date. The reconstituted antibody is stable for at least two months when stored at 4¡ãC. For long term storage, it is recommended that aliquots of the antibody are frozen at -20¡ãC (frost-free freezers are not recommended). Repeated freezing and thawing must be avoided. Prepare working dilutions on the day of use.

Storage Buffer
Theammonium sulfate-precipitated ascites fluid is in PBS + 1% BSA & then lyophilized.

Type
Mouse Antibody Monoclonal.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Westren Blotting

Antigen
Recommended: working dilution on Western bloting 1:500. 60 minutes primary antibody incubates at 25¡ãC.