Matrix Metalloproteinase-13 Human Recombinant, MMP 13 Human, 2.5ug

CAT No.BT-ENZ-317

Matrix Metalloproteinase-13 Human Recombinant, MMP 13 Human, 2.5ug

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Applications
Used as a standard for assaying MMP-13 or for screening inhibitors.

Biological Activity
Activity is determined by the cleavage of fluorogenic peptide, 100 ng of enzyme activated with APMA will digest 75-80% (1.5-1.6 nmole) of fluorogenic peptide substrate (0.1ml of 20?M solution) at 35¡ãC for 30 minutes.

Description
Matrix Metalloproteinase-13 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain having a molecular mass of 27 kDa.
The Collagenase 3 is purified by proprietary chromatographic techniques.

The protein Solution (100 ug/ml) in 0.05M Tris-HCl buffer, pH 7.6, containing 0.2M NaCl, 5mM CaCl2, 20?M ZnSO4 and 0.1% BSA and NaN3.

Physical Appearance
Sterile Filtered clear solution.

Purity
Greater than 90% as determined by SDS-PAGE.

Source
Escherichia Coli.

Stability
MMP-13 although stable at 4¡ãC for 1 week, should be stored desiccated below -18¡ãC.
Please prevent freeze-thaw cycles.

Synonyms
Collagenase 3, EC 3.4.24.-, Matrix metalloproteinase-13, MMP-13, CLG3.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.