Mitogen Activated Kinase Kinase 1 Human Recombinant, MEK1 Human, 5ug

CAT No.BT-PKA-256

Mitogen Activated Kinase Kinase 1 Human Recombinant, MEK1 Human, 5ug

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Formulation
MEK1 is supplied at a concentration of 0.09mg/ml in 40mM Tris, pH-8, 0.15M NaCl, 0.27M sucrose, 1mM DTT, 0.2mM PMSF, 1mM benzamidine, 0.1mM sodium vanadate and 0.03% Brij-35.

Biological Activity
~125-175 units/mg. One unit of MEK1 activity transfers 1 nmol of phosphate to ERK1/2 peptide per minute at 30¡ãC in a reaction containing 100?M ATP.
Recombinant active MEK1 also phosphorylates ERK1, ERK2, and GSK-3?. Kinase activity may vary depending on the substrate and reaction conditions.
The optimal concentration should be determined for each specific application.

Description
MAP2K1 active Human Recombinant produced in Sf9 cells is a glycosylated, polypeptide chain containing amino acids 2-393 having a molecular mass of 47 kDa. MAP2K1 is fused to a polyhistidine tag and is purified by proprietary chromatographic techniques.

Sterile Filtered clear solution.

Purity
Greater than 90% as determined by SDS-PAGE.

Source
Sf9 Insect Cells.

Storage Conditions
Store at 4¡ãC if entire vial will be used within 1-2 weeks.
Store frozen at -20¡ãC for longer periods of time.
Avoid multiple freeze-thaw cycles.

Synonyms
MEK1, MKK1, MAPKK1, MAP2K1, Dual specificity mitogen-activated protein kinase kinase 1, MAP kinase kinase 1, EC 2.7.12.2, ERK activator kinase 1, MAPK/ERK kinase 1, PRKMK1.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.