Mouse NIH 3T3+Vanadate+PDGF Lysate, 500ug

Important note: Products are intended for laboratory use and are not to be used for any other purpose, which include but are not limited to, unauthorized commercial uses, ex vivo or in vivo therapeutic uses. Not for Human or Animal Consumption.

Cat NO. CL047

Mouse NIH 3T3+Vanadate+PDGF Lysate

   FOR RESEARCH USE ONLY

Volume: 500 ?g                                    

l  Storage

?        Store undiluted at -20 ?C for long term storage

?        Store undiluted at 4 ?C for short time storage (up to one month)

?        Stable at RT up to one week

?        Do not dilute or boil

l  Description

Cell or tissue lysates are a useful control for the western blotting application. As a source that can be rich in endogenously expressed proteins, this lysate is convenient to be used and provided in a denatured format and allowing for immediately loading onto an SDC-PAGE gel.

This lysate was routinely tested by western blot analysis.

 

l  Preparation

NIH 3T3 cells were treated by serum starved overnight and then incubated at RT for 10 min in a final concentration of 1 mM sodium vanadate. PDGF was then added at a final concentration of 5 ng/ml and cells were incubated at 37 ?C for 30 min. Western blots of NIH 3T3 cell lysates treated with vanadate and PDGF have been reported to show a shift in SHC mobility in addition to phosphorylation of PKBα/Akt and Stat3 as compared to untreated control cells.

 

l  Application

Western Blot

 

l  Product Notice

Due to variable applications, each investigator should titrate the reagent to obtain optimal results.