Mouse Ultrasensitivity Tri-iodothyronine,u-T3 ELISA Kit, 96T

Mouse Ultrasensitivity Tri-iodothyronine (u-T3) ELISA Kit, 96T

Assay range: 0.25 – 16 ng/ml                                       96 Determinations

Purpose

This kit is used for the quantitative determination the concentrations of Mouse Ultrasensitivity Tri-iodothyronine (u-T3) in mouse serum, plasma, cell culture supernatants, and other biological fluids.

Principle of the assay

The kit assays mouse u-T3 in the samples by the method of biotin–avidin mediated competitive ELISA. The solid-phase antibody is prepared by purified mouse u-T3 antibody which is pre-coated onto a microplate. After loading of samples, the u-T3 in the samples or the biotinylated u-T3 competitively bind with the coated antibody. Then the HRP-Streptavidin enzyme conjugate has been added into wells. After washing to remove any unbound reagent, the tetramethylbenzidine (TMB) substrate is added. TMB substrate produces blue color when catalyzing by the HRP enzyme. After reaching the desired color intensity, the reaction is terminated by addition of an acidic Stop solution which changes the solution color from blue to yellow. Determining the absorbance (OD Value) at wave length 450 nm by the microwell reader, and the concentration of u-T3 can be calculated according to standard curve.

Kit Performance

1.    Sensitivity: less than 0.25 ng/ml (0.38 nmol/L).

2.  Specificity: No cross-reaction with T4, rUT3.

3.   Repeatability: both inter-assay and intra-assay were less than 12 %.

4.   Samples from hematolysis or Chyle blood may affect the accuracy.

*Lead time of this kit is 1-2 weeks. Shipped by FedEx air. 
*Storage: 2 - 8 C. Shelf Time: 6 months.
*Kit Experimental Time:< 1 hs

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Important note: Products are intended for laboratory or manufacture use and are not to be used for diagnosis or any other purpose, which include but are not limited to, unauthorized commercial uses, ex vivo or in vivo therapeutic uses. Not for Human or Animal Consumption.