Neurotrophin-3 Human Recombinant, NT 3 Human, 10ug

Amino acid sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Tyr-Ala-Glu-His-Lys.

Biological Activity
The ED50 as determined by the dose-dependant induction of choline acetyl transferase in rat basal forebrain primary septal culture was found between 20-50ng/ml corresponding to a Specific Activity of 20,000-50,000IU/mg.

Description
Neurotrophin-3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 119 amino acids and having a molecular mass of 13606.29 Dalton.
The NT-3 is purified by proprietary chromatographic techniques.

Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

Protein content
Protein quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 2.165 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

2. Analysis by RP-HPLC, using a standard solution of NT-3 as a Reference Standard.

Purity
Greater than 97.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility
It is recommended to reconstitute the lyophilized Neurotrophin-3 in sterile 18M¦¸-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source
Escherichia Coli.

Stability
Lyophilized NGF2 although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution NGF-2 should be stored at 4¡ãC between 2-7 days and for future use below
-18¡ãC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms
Neurotrophic factor, Nerve growth factor-2, NGF-2, HDNF, NT-3.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.