Parvalbumin Rat Recombinant, PVALB Rat, 10ug

CAT No.BT-PRO-398

Parvalbumin Rat Recombinant, PVALB Rat, 10ug

---

Applications
The Parvalbumin can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection.
For fluid-phase adsorption in immunohistochemistry we suggest the following procedure: A- Dilute 1 ?l of the monoclonal antibody 235 against parvalbumin in 5 ml of the usual buffer for immunohistochemistry (final dilution 1:5'000). B- Add 1ug of the Parvalbumin to 1ml of the diluted antibody solution and mix well. C- Incubate for at least 6 hours in the cold. D- Apply to tissue-sections and incubate for 3 days. D- Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB). As a result, the immunostaining should be strongly reduced or even completely prevented.

Description
Recombinant Rat Parvalbumin produced in E.Coli.
The Rat Parvalbumin is purified by proprietary chromatographic techniques.

Formulation
The protein was lyophilized from a concentrated solution (1mg/ml) containing 10mM Tris-base, 70mM NaCl, 1mM EDTA and 1mM b-mercaptoethanol, pH 7.4.

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity
Greater than 90% as determined by SDS-PAGE.

Solubility
It is recommended to reconstitute the lyophilized Parvalbumin in sterile 18M¦¸-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source
Escherichia Coli.

Stability
Lyophilized Parvalbumin although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution Parvalbumin should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms
Pvalb, Pva, PALB1, Parvalbumin.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.