Placental Ovine Growth Hormone Recombinant, GH Ovine, Placental, 200ug

CAT No.BT-CYT-594

Placental Ovine Growth Hormone Recombinant, GH Ovine, Placental, 200ug

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Amino acid sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Thr-Phe-Pro-Ala.

Biological Activity
Ovine placental Grwoth Hormone is fully biologically active when compared to World Health Organization (WHO) reference standard using in vitro bioassay in PDF-P1 3B9 cells stably transfected with rabbit GH receptors. It is also capable of forming a 1:2 complex with the recombinant ovine growth hormone receptor extracellular domain (ECD).

Description
Placental Growth Hormone Ovine Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 191 amino acids and having a molecular mass of 21918 Dalton.
The GH Ovine Recombinant is purified by proprietary chromatographic techniques.
Placental Growth Hormone differs from pituitary Ovine Growth Hormone by two amino acids G9R/G63S. Placental Ovine Growth Hormone possesses higher biological activity as compared to pituitary Ovine GH.

The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3 adjusted to pH-9.

Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

Protein content
Protein quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 0.684 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of GH as a Reference Standard.

Purity
Greater than 98.0% as determined by:
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.

Solubility
It is recommended to reconstitute the lyophilized Growth Hormone in sterile 0.4% NaHCO3 pH-9 not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source
Escherichia Coli.

Stability
Lyophilized Ovine Placental Growth Hormone although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution GH Ovine Placental should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms
GH1, GH, GHN, GH-N, hGH-N,Pituitary growth hormone, Growth hormone 1, Somatotropin.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.