Polyphosphate Kinase, PPK, 100IU

CAT No.BT-ENZ-266

Polyphosphate Kinase, PPK, 100IU

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Definition of Activity Units
The amount of Polyphosphate kinase required to convert 1 ?mole ADP to ATP per minute at pH 7.5, using polyphosphate as phosphate donor.

Description
Polyphosphate Kinase purified circa 10 fold . Free of all Polyphosphate Glucokinase activity.
Native MW = 83 kD (monomer).

Formulation
The protein was lyophilized from 1.15ml PPK solution containing 43.6 U/ml of PPK activity, 0.77 mg/ml total protein, 10mM potassium phosphate pH 6.8 and 25mM sodium polyphosphate.

Sterile Filtered White lyophilized (freeze-dried) powder.

Required Cofactors
The reaction requires Mg2+.

Solubility
The contents of the vial can be reconstituted in 1-10 ml deionized water.

Source
Propionibacterium shermanii.

Specific Activity
56.5 U/mg.

Stability
Lyophilized Polyphosphate kinase although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution PPK should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms
Polyphosphate kinase, EC 2.7.4.1, Polyphosphoric acid kinase, ATP-polyphosphate phosphotransferase.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.