Progranulin Human Recombinant, PGRN Human, 10ug

CAT No.BT-CYT-524

Progranulin Human Recombinant, PGRN Human, 10ug

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Stability
Progranulin although stable at 4¡ãC for 3 weeks, should be stored at -20¡ãC for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Biological Activity
1. The effects of phospho-ERK1/2 and non phospho-ERK1/2 by human progranulin-FLAG in THP-1 cells.<br />To examine the signal of phospho-p44/42 MAPK and p44/42 MAP kinase, reactions were carried out at 37 degrees Celsius over 0, 30, 60, minutes, respectively by adding the recombinant protein (100ng/ml corresponding to a Specific Activity of 100IU/mg) to the THP-1 monocyte cell, which was maintained with serum starvation for 24hrs. Recombinant proteins in lanes 1, 2, and 3 were subjected to THP-1 monocyte cell treatments over 0, 30, 60mins, respectively.<br />2. The effects of phospho-ERK1/2 and non phospho-ERK1/2 by human progranulin-FLAG in MCF10A cells. To examine the signal of phospho-p44/42 MAPK and p44/42 MAP kinase, reactions were carried out at 37 degrees Celsius over 0, 10, 30, 60mins, respectively by adding the recombinant protein(500ng/ml) to the MCF10A human breast epithelial cells, which was maintained with serum starvation for 24hrs. Recombinant proteins in lanes 2, 3, and 4 were subjected to MCF10A human breast epithelial cell treatments over 0, 10, 30, 60mins, respectively.

Description
Progranulin Human Recombinant fused to FLAG at C-terminus produced in HEK is a single, glycosylated, polypeptide chain containing 593 amino acids and having a molecular mass of 88 kDa.
The Progranulin is purified by standard chromatographic techniques.

The protein solution contains 1x PBS.

Physical Appearance
Sterile Filtered clear solution.

Purity
Greater than 90.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Source
HEK 293 cells.

Synonyms
GRN, PGRN, granulin, Acrogranin, propithelin, PC cell derived growth Factor, GEP, GP88, PEPI, PCDGF.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.