Prolactin Soluble Receptor Bovine Recombinant, PRL R Bovine, 50ug

CAT No.BT-CYT-294

Prolactin Soluble Receptor Bovine Recombinant, PRL R Bovine, 50ug

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Amino acid sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Gln-Ser-Pro-Pro-Glu.

Description
Prolactin Receptor Bovine Extra Celleular Domain Recombinant ?produced in E.Coli is a non-glycosylated, Polypeptide chain containing 213 amino acids and having a molecular mass of 24.4 kDa.
The Prolactin Receptor is purified by proprietary chromatographic techniques.

Formulation
The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3.

Sterile Filtered White lyophilized (freeze-dried) powder.

Protein content
Protein quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 2.7 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

2. Analysis by RP-HPLC, using a standard solution of PRL-R as a Reference Standard.

Purity
Greater than 97.0% as determined by:
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.

Solubility
It is recommended to reconstitute the lyophilized
PRL-R in sterile 18M¦¸-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source
Escherichia Coli.

Stability
Lyophilized PRL-R although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution Prolactin Receptor should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms
PRL-R, PRLR, OPR, PRLrI.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.