Protein Tyrosine Phosphatase Non Receptor Type-1 Human Recombinant, PTPN1 Human, 50ug

CAT No.BT-PKA-219

Protein Tyrosine Phosphatase Non Receptor Type-1 Human Recombinant, PTPN1 Human, 50ug

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Amino Acid Sequence
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Biological Activity
Specific activity: 6,000-12,000units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37C, pH7.5 using 10mM of substrate.

Description
Protein Tyrosine Phosphatase Non Receptor Type-1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 321 amino acids and having a molecular mass of 37.3 kDa.

The protein contains 25mM Tris-HCl, pH 7.5, 2mM b-mercaptoethanol, 1mM EDTA, 1mM DTT and 20% Glycerol.

Physical Appearance
Sterile filtered colorless solution.

Purity
Greater than 95.0% as determined by SDS-PAGE.

Source
Escherichia Coli.

Stability
Store at 4¡ãC if entire vial will be used within 2-4 weeks.
Store, frozen at -20¡ãC for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.

Synonyms
Tyrosine-protein phosphatase non-receptor type 1, EC 3.1.3.48, Protein-tyrosine phosphatase 1B, PTP-1B, PTPN1, PTP1B.

Unit Definition
One unit will hydrolyze 1 nanomole of p-nitrophenylphosphatate per minute at pH 7.4 at 37¡ãC using 10mM of substrate.

Usage
TSZGENE's products are furnished for LABORATORY ESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.