Rat 5-hydroxyindolacetic acid,5-HIAA ELISA Kit, 96T

Rat 5-hydroxyindolacetic acid,5-HIAA ELISA Kit, 96T

Assay range: 1.5 – 400 ng/ml                                   

Purpose

This kit is used for the quantitative determination the concentrations Rat 5-hydroxyindolacetic acid (5-HIAA) in rat serum, plasma, cell culture supernatants, and other biological fluids.
Principle of the assay

The kit assays 5-HIAA in the rat samples by the method of colorimetric ELISA. After loading of samples, the 5-HIAA in the standards or samples interacts with TMB-substrate solution. After incubation, the reaction is terminated by addition of Stop solution. Determining the absorbance (OD Value) at wave length 450 nm by the microwell reader, and the concentration of 5-HIAA can be calculated according to standard curve.

Kit Performance                                   

1.    Sensitivity: less than 1.5 ng/ml.

2.  Specificity: simultaneous detection of recombinant or natural 5-HIAA without cross-reaction with other cytokines.

3.   Repeatability: both inter-assay and intra-assay were less than 10 %.

*Lead time of this kit is 1-2 weeks. Shipped by FedEx air. 
*Storage: 2 - 8 C. Shelf Time: 6 months.
*Kit Experimental Time:< 2 hs

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Important note: Products are intended for laboratory or manufacture use and are not to be used for diagnosis or any other purpose, which include but are not limited to, unauthorized commercial uses, ex vivo or in vivo therapeutic uses. Not for Human or Animal Consumption.