RELM-Beta Human Recombinant, RELM b Human, 25ug

Applications
Western blotting.

Description
RELM-beta Human Recombinant is a His-Tagged Fusion Protein which is 11 kDa protein containing 90 amino acid residues of the RELM-beta human and 12 additional amino acid residues - HisTag (underlined).
MGSTQCSLDS VMDKKIKDVL NSLEYSPSPI SKKLSCASVK SQGRPSSCPA GMAVTGCACG YGCGSWDVQLETTCHCQCSV VDWTTARCCH LTKLRSHHHH HH.

Formulation
Sterile filtered and lyophilized from 0.5 mg/ml in 0.05M Acetate buffer pH-4.

Purification Method
Three-step procedure using affinity Ni-NTA chromatography and size exclusion chromatography before and after refolding.

Purity
Greater than 95% as determined by SDS-PAGE.

Solubility
Add 0.2 ml of 0.1M Acetate buffer pH-4 and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10¦Ìg/ml. In higher concentrations the solubility of this antigen is limited.

Source
Escherichia Coli.

Specificity
The amino acid sequence of the RELM-beta human recombinant is 100% homologous to the amino acid sequence of the RELM-beta human without signal sequence.

Stability
Store lyophilized protein at -20¡ãC. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4¡ãC for a limited period of time; it does not show any change after two weeks at 4¡ãC.
The lyophilized protein remains stable until the expiry date when stored at -20¡ãC.

Synonyms
Resistin-like beta, RELM beta, Cysteine-rich secreted protein FIZZ2, Colon and small intestine-specific cysteine-rich protein, Cysteine-rich secreted protein A12-alpha-like 1, Colon carcinoma-related gene protein, RELM-b, XCP2, HXCP2.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.