Retinol Binding Protein-4 Human Recombinant, His tag, RBP4 Human, His, 10ug

CAT No.BT-CYT-555

Retinol Binding Protein-4 Human Recombinant, His tag, RBP4 Human, His, 10ug

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Biological Activity
Determined by its ability to bind all trans retinol acids. The binding of RBP4 to all trans retinol acids results in quenching of Trp fluroscence. 0.5M retinol acids/per M RBP4.

Amino acid sequence
MSWWHHHHHH NWNIPTTQDT TERDCRVSSF RVKENFDKAR FSGTWYAMAK KDPEGLFLQD NIVAEFSVDE TGQMSATAKG RVRLLNNWDV CADMVGTFTD TEDPAKFKMK YWGVASFLQK GNDDHWIVDT DYDTYAVQYS CRLLNLDGTC ADSYSFVFSR DPNGLPPEAQ KIVRQRQEEL CLARQYRLIV HNGYCDGRSERNLL.

Description
RBP-4 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 204 amino acids and having a molecular mass of 23 kDa.
RBP4 is fused to N-terminus His tag which containes additional 21 amino acids. RBP4 sequence is identical to UniProtKB/Swiss-Prot entry Q5VY30 amino acids 16¨C199.
The Retinol Binding Protein-4 is purified by proprietary chromatographic techniques.

RBP-4 His tag was filtered (0.4 um) and lyophilized from 0.5 mg/ml in PBS buffer, pH 7.5.

Physical Appearance
White lyophilized (freeze-dried) powder.

Purity
Greater than 95.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility
It is recommended to reconstitute the lyophilized Retinol binding protein-4 in sterile deionized water to a working volume of 0.5mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

Source
Escherichia Coli.

Stability
Lyophilized Retinol binding protein-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution Retinol binding protein-4 should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms
Retinol Binding Protein 4 plasma, RBP-4, RBP4, Plasma retinol-binding protein, PRBP, RBP.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.