Secreted Phospholipase A2-IIE Human Recombinant, PLA2G2E Human, 10ug

CAT No.BT-ENZ-327

Secreted Phospholipase A2-IIE Human Recombinant, PLA2G2E Human, 10ug

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Applications
Western blotting.

Description
Secreted Phospholipase A2-IIE Human Recombinant manufactured with N-terminal His-Tag. PLA2G2E His-Tagged Fusion Protein is 15.8 kDa protein containing 123 amino acid residues of the human secreted phospholipase A2-IIE and 16 additional amino acid residues ¨C His-Tag (underlined).
MRGSHHHHHHGMASHMNLVQFGVMIEKMTGKSALQYNDYGCYCGIGGSH
WPVDQTDWCCHAHDCCYGRLEKLGCEPKLEKYLFSVSERGIFCAGRTTCQR
LTCECDKRAALCFRRNLGTYNRKYAHYPNKLCTGPTPPC.

Formulation
Sterile filtered and lyophilized from 0.5 mg/ml in 0.05M Acetate buffer pH-4.

Sterile Filtered lyophilized (freeze-dried) powder.

Purification Method
Ni-NTA affinity chromatography.

Purity
Greater than 95% as determined by SDS PAGE.

Solubility
Add 0.2 ml of 0.1M Acetate buffer pH-4 and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10 ¦Ìg/ml. In higher concentrations the solubility of this antigen is limited.

Source
Escherichia Coli.

Specificity
The amino acid sequence of the recombinant human Secreted Phospholipase A2-IIE is 100% homologous to the amino acid sequence of the human Secreted Phospholipase A2-IIE without signal sequence.

Stability
Store lyophilized protein at -20¡ãC. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4¡ãC for a limited period of time; it does not show any change after two weeks at 4¡ãC.
The lyophilized protein remains stable until the expiry date when stored at -20¡ãC.

Synonyms
Group IIE secretory phospholipase A2, EC 3.1.1.4, Phosphatidylcholine 2-acylhydrolase GIIE, GIIE sPLA2, sPLA(2)-IIE, sPLA2-IIE, PLA2G2E.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.