Stem Cell Factor Human Recombinant, Sf9, SCF Human, Sf9, 10ug

CAT No.BT-CYT-421

Stem Cell Factor Human Recombinant, Sf9, SCF Human, Sf9, 10ug

---

Biological Activity
The ED50 as determined by the dose-dependant stimulation of Human TF-1 cells is typically 2-5ng/ml corresponding to a Specific Activity of 0.2-0.5MIU/mg.

Description
Stem Cell Factor Human Recombinant produced in insect cells is a single, glycosylated polypeptide chain containing 165 amino acids and having a molecular mass of 18409 Dalton.
The SCF is fused to a C-terminal His-tag (6x His) and purified by proprietary chromatographic techniques.

Formulation
The protein is supplied in 1X PBS, pH 7.4.

Sterile Filtered White lyophilized (freeze-dried) powder.

Protein content
Protein quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 0.52 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of Stem Cell Factor as a Reference Standard.

Purity
Greater than 95.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility
It is recommended to reconstitute the lyophilized Stem Cell Factor in 10 mM acetic acid not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source
Sf9, Insect Cells.

Stability
Lyophilized KIT ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution SCF should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms
Kit ligand Precursor, C-kit ligand, SCF, Mast cell growth factor, MGF, SF, KL-1, Kitl, DKFZp686F2250.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.