Streptavidin, Streptavidin, 10mg

CAT No.BT-PRO-283

Streptavidin, Streptavidin, 10mg

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Application
Streptavidin may be used to visualize biotin conjugated molecules in ELISA, blotting and histological technique.

Description
The Streptavidin preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogeneity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket as compared to native streptavidin. Streptavidin has a molecular weight of 55kDa.

Formulation

Lyophilized (1mg/ml) in 50mM NaCl, pH 9.0.

Sterile Filtered lyophilized powder.

Proteolytic Activity
Less than 10-3 U/mg protein (Azocoll, 25¡ãC, 24 h, pH 8.0).

Purity
Greater than 95.0% as determined by SDS-PAGE.

Solubility
Gives a clear solution at 5mg/ml in 0.1M NaCl.

Source
Bacterium Streptomyces avidinii.

Specific Activity
Greater than 15.7 U/mg, 1 unit binds 1ug biotin.

Stability
Streptavidin although stable at 4¡ãC for 3 weeks, should be stored desiccated below -18¡ãC.
For longer storage in dissolved form add 1mM EDTA and/or 0.02 % NaN3 or pass the solution through a sterile filter.
Please prevent freeze-thaw cycles.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.