TIE2 Fc Chimera Human Recombinant, TIE2 Fc Human, 10ug

CAT No.BT-PKA-248

TIE2 Fc Chimera Human Recombinant, TIE2 Fc Human, 10ug

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Biological Activity
Measured in a functional ELISA assay. When TIE-2/Fc is immobilized at 4 ug/mL (100 ?l/well), it binds rh Angiopoietin-2 with a linear range of 2 -100 ng/ml.

Description
Soluble TIE-2 Human Recombinant fused with the Fc part of human IgG1 produced in baculovirus is a monomeric, glycosylated, polypeptide containing 730 amino acids and having a total molecular mass of 250 kDa. Human TIE-2/Fc monomer has a calculated molecular mass of approximately 125 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 140 kDa protein in SDS-PAGE under reducing conditions.
The TIE2 Fc Chimera is purified by proprietary chromatographic techniques.

Formulation
TIE-2 Fc Chimera was lyophilized from a concentrated (1 mg/ml) sterile solution containing 1x PBS.

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity
Greater than 90.0% as determined by(a)Analysis by RP-HPLC.
(b)Analysis by SDS-PAGE.

Solubility
It is recommended to reconstitute the lyophilized TIE-2 Fc Chimera in sterile water not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source
Insect Cells.

Stability
Lyophilized sTIE-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution TIE-2 should be stored at 4¡ãC between 2-7 days and for future use below
-18¡ãC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms
Angiopoietin-1 receptor precursor, Tyrosine-protein kinase receptor TIE-2, hTIE2, Tyrosine-protein kinase receptor TEK, p140 TEK, Tunica interna endothelial cell kinase, CD202b, VMCM, VMCM1, TIE2.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.