Tobacco Etch Virus Protease Recombinant, TEV, 500IU

CAT No.BT-PRO-585

Tobacco Etch Virus Protease Recombinant, TEV, 500IU

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Activity
10,000 Units/1mg.

Cleavage Conditions
A number of variables can be changed to optimize the cleavage of any specific protein. The amount of rTEV, the temperature of the incubation, and the time needed for cleavage may be examined. If the protein of interest is heat-labile, then 4¡ãC incubations are recommended. Reactions at 4¡ãC will require longer incubation times and/or more rTEV.

Description
Recombinant TEV Protease (rTEV) is a site-specific protease purified from E. coli. The protease can be used for the removal of affinity tags from fusion proteins. The seven-amino-acid recognition site for rTEV is Glu-Asn-Leu-Tyr-Phe-Gln-Gly with cleavage occurring between Gln and Gly. The optimal temperature for cleavage is 30¡ãC; however, the enzyme can be used at temperatures as low as 4¡ãC.
The rTEV contains His tag.
The rTEV is purified by proprietary chromatographic techniques.

The rTEV contains 0.50 M Tris-HCl pH 8.0, 10mM DTT and 5 mM EDTA.

Physical Appearance
Sterile liquid formulation.

Purity
Greater than 90.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Source
Escherichia Coli.

Stability
Store rTEV at -70?C for long term or at -20?C for< 6 months. Please prevent freeze-thaw cycles.

Synonyms
rTEV, TEV, P1 protease.

Unit Definition
One unit of rTEV cleaves >85% of 3 ug control substrate in 1 h at 30¡ãC.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.