Transforming Growth Factor-beta 1 Human, TGF b 1 Human, 2.5ug

CAT No.BT-CYT-561

Transforming Growth Factor-beta 1 Human, TGF b 1 Human, 2.5ug

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Biological Activity
Stimulates the growth of NRK-1 cells in soft agar at concentrations ranging from 0.1 to 5ng/ml corresponding to a specific activity of 0.2-10MIU/mg. Effective concentration ranges must be experimentally determined. Purified EGF and/or TGF-alpha at concentrations of at least 2ng/ml must be present for observation of the biological activity.

Description
Human Transforming Growth Factor-beta 1 purified from Human Platelets having a molecular mass of 25kDa.
The TGF-b 1 is purified by proprietary chromatographic techniques.

Formulation
TGF-Beta1 protein was lyophilized from a solution containing 5mM Sodium Citrate (200?l) pH 3.5.

Sterile Filtered lyophilized powder.

Purity
Greater than 97.0%.

Solubility
It is recommended to reconstitute lyophilized TGF-beta 1 in sterile 100?l 5mM HCl, which can then be further diluted to the desired aliquot to 1ml of PBS containing 2mg/ml Albumin.

Source
Human Platelets.

Stability
Lyophilized TGF-beta 1 although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution TGF-beta 1 should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC.
DO NOT RECONSTITE WITH NEUTRAL BUFFERS.
DO NOT USE GLASS IMPLEMENTS OR EXTENSIVE MANIPULATIONS.
PREVENT FREEZE THAW CYCLES.

Synonyms
Transforming growth factor beta-1, TGF-beta-1, CED, DPD1, TGFB, TGF-b 1.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.