Trefoil Factor-3 Human Recombinant, TFF3 Human, 20ug

CAT No.BT-CYT-005

Trefoil Factor-3 Human Recombinant, TFF3 Human, 20ug

---

Amino acid sequence
EEYVGLSANQ CAVPAKDRVD CGYPHVTPKE CNNRGCCFDS RIPGVPWCFK
PLQEAECTF

Biological Activity
Determined by its ability to chemoattract human MCF-7 cells using a concentration 1.0-10.0 ng/ml.

Description
TFF-3 Human Recombinant produced in E.Coli is a homodimeric, non-glycosylated, polypeptide chain containing 2 x 59 amino acid chains which includes a 40 amino acid trefoil motif containing 3 conserved interamolecular disulfide bonds and having a total molecular mass of 13.2kDa.
TFF-3 Human Recombinant is purified by proprietary chromatographic techniques.

Lyophilized from a 0.2?m filtered concentrated solution in PBS, pH 7.4.

Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

Purity
Greater than 97.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility
It is recommended to reconstitute the lyophilized TFF3 in sterile 18M?-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source
Escherichia Coli.

Stability
Lyophilized TFF3 although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution TFF3 should be stored at 4¡ãC between 2-7 days and for future use below
-18¡ãC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms
TFF-3, ITF, TFI, HITF, hP1.B, TFF3, Trefoil factor 3, Intestinal trefoil factor.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.