Trypsin Human Recombinant, Trypsin Human, 5mg

CAT No.BT-PRO-770

Trypsin Human Recombinant, Trypsin Human, 5mg

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Biological Activity
10,000 BAEE units/mg powder.

Description
Recombinant Human Trypsin-2 is free from any animal and human sources. Recombinant Human Trypsin-2 expressed in E.Coli having a Mw of 24kDa is purified by standard chromatography techniques. Recombinant Human Trypsin-2 is free from foreign enzymes such as carboxypeptidase A & chymotrypsin. Recombinant Human Trypsin-2 is free from protease inhibitors such as PMSF and EDTA.

Formulation
The protein was lyophilized without 10mM Sodium Acetate buffer and 50mM NaCl.

Sterile Filtered lyophilized powder.

Purity
Greater than 90% as determined by SDS-PAGE.

Solubility
It is recommended to reconstitute the lyophilized Human Trypsin in sterile 18M¦¸-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source
Escherichia Coli.

Stability
Recombinant Human Trypsin although stable at room temp for 1 week, should be stored desiccated below -18?C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Unit Definition
One BAEE unit will produce a Delta A253 of 0.001 per minute in a reaction volume of 3.0ml at pH7.6 at 25?C (1cm light path). Trypsin digestion: suggested ratio (w:w) 1:20 to 1:1000 (Trypsin : Peptide).

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.